03208nam a22004337a 4500 OSt 20250930111024.0 250930b |||||||| |||| 00| 0 eng d University of Cebu-Banilad University of Cebu-Banilad Abejo, Joshua David S., author. In vitro anti-quorum sensing activity of terminalia catappa fruit extract against serratia marcescens / Joshua David S, Abenjo [and nine others]. Cebu City, Philippines : University of Cebu-Banilad, c2025. 63 pages : colored illustration ; 12 cm. rdacontent text rdamedia unmediated rdacarrier volume Incudes bibliographical references. "Quorum sensing (QS) is a bacterial communication process that regulates biofilm formation and virulence, with recent research focusing on inhibiting QS to control antibiotic-resistant infections. This study investigates the anti-QS activity of Terminalia catappa fruit extract against Serratia marcescens, an opportunistic pathogen linked to hospital-acquired infections. By exploring the potential of T. catappa fruit extract to inhibit QS-regulated behaviors such as pigment production, this research aims to contribute to the development of alternative antibacterial strategies and reduce reliance on conventional antibiotics. T. catappa fruit extract was obtained using Soxhlet extraction with 95% ethanol, followed by rotary evaporation. MIC and MBC were determined via microdilution. Anti-quorum sensing activity was assessed via 96-well microplate assay. Statistical analysis was performed using one-way ANOVA and Tukey-Kramer post hoc analysis. The extract showed weak antimicrobial and quorum-sensing inhibition against S. marcescens. The MIC was 250 mg/mL with no significant bactericidal effect. At this concentration, the extract inhibited 46.69% of prodigiosin production, indicating moderate QS inhibition. The 4.5% extraction yield suggests limited potency. While previous studies highlight the antibacterial effects of leaf extracts, the fruit extract demonstrated limited efficacy. Future studies should explore the mechanisms underlying T. catappa extract's antimicrobial and quorum-sensing activities." --Abstract Adult Apuhin, Mercy Milagros College of Nursing Nursing Apuhin, Mercy Milagros College of Nursing Nursing Text in English Quorum-sensing activities. Fruit extract. Serratia marcescens. Ambor, Sittie Alaicah U., author. Castañares, Mari Tonette J., author. Cubay, Exxon Z., author. Emborgo, Rikk Daniel, author. Flores, Ria Ariadne T., author. Jayubo, Rochelle B., author. Lim, Doni Corinne E., author. Priagula, Merch Issa R., author. Salgados, Peter Gabriel B., author. ddc THE Janna [new] 09/30/2025 Janna [added] 09/30/2025 14489 14489 0 0 ddc 0 0 UCBL_MED UCBL_MED PERIODICALS 2025-09-30 T Ab33v 2025 3UCSM000001566 2025-09-30 c.1 2025-09-30 THE 0 0 ddc 0 0 UCBL_MED UCBL_MED PERIODICALS 2025-09-30 0 T Ab33v 2025 3UCSM000001567 2025-09-30 c.2 2025-09-30 THE