000			03208nam a22004337a 4500
003			OSt
005			20250930111024.0
008			250930b |||||||| |||| 00| 0 eng d
040			_aUniversity of Cebu-Banilad _cUniversity of Cebu-Banilad
100			_aAbejo, Joshua David S., _eauthor.
245			_aIn vitro anti-quorum sensing activity of terminalia catappa fruit extract against serratia marcescens / _cJoshua David S, Abenjo [and nine others].
260			_aCebu City, Philippines : _bUniversity of Cebu-Banilad, _cc2025.
300			_a63 pages : _bcolored illustration ; _c12 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncudes bibliographical references.
520			_a"Quorum sensing (QS) is a bacterial communication process that regulates biofilm formation and virulence, with recent research focusing on inhibiting QS to control antibiotic-resistant infections. This study investigates the anti-QS activity of Terminalia catappa fruit extract against Serratia marcescens, an opportunistic pathogen linked to hospital-acquired infections. By exploring the potential of T. catappa fruit extract to inhibit QS-regulated behaviors such as pigment production, this research aims to contribute to the development of alternative antibacterial strategies and reduce reliance on conventional antibiotics. T. catappa fruit extract was obtained using Soxhlet extraction with 95% ethanol, followed by rotary evaporation. MIC and MBC were determined via microdilution. Anti-quorum sensing activity was assessed via 96-well microplate assay. Statistical analysis was performed using one-way ANOVA and Tukey-Kramer post hoc analysis. The extract showed weak antimicrobial and quorum-sensing inhibition against S. marcescens. The MIC was 250 mg/mL with no significant bactericidal effect. At this concentration, the extract inhibited 46.69% of prodigiosin production, indicating moderate QS inhibition. The 4.5% extraction yield suggests limited potency. While previous studies highlight the antibacterial effects of leaf extracts, the fruit extract demonstrated limited efficacy. Future studies should explore the mechanisms underlying T. catappa extract's antimicrobial and quorum-sensing activities." --Abstract
521			_aAdult
541			_xApuhin, Mercy Milagros _yCollege of Nursing _zNursing
541			_xApuhin, Mercy Milagros _yCollege of Nursing _zNursing
546			_aText in English
650			_aQuorum-sensing activities.
650			_aFruit extract.
650			_aSerratia marcescens.
700			_aAmbor, Sittie Alaicah U., _eauthor.
700			_aCastañares, Mari Tonette J., _eauthor.
700			_aCubay, Exxon Z., _eauthor.
700			_aEmborgo, Rikk Daniel, _eauthor.
700			_aFlores, Ria Ariadne T., _eauthor.
700			_aJayubo, Rochelle B., _eauthor.
700			_aLim, Doni Corinne E., _eauthor.
700			_aPriagula, Merch Issa R., _eauthor.
700			_aSalgados, Peter Gabriel B., _eauthor.
942			_2ddc _cTHE
998			_cJanna [new] _d09/30/2025
998			_cJanna [added] _d09/30/2025
999			_c14489 _d14489