000			04058nam a22005297a 4500
003			OSt
005			20260921095021.0
008			260918b |||||||| |||| 00| 0 eng d
040			_aUniversity of Cebu - School of Medicine _cUniversity of Cebu - School of Medicine
100			_aGalano, Ralph Dominic C., _eauthor.
245			_aAnti-quorum sensing activity of Psidium guajava L. methanolic leaf extracts against Pseudomonas aeruginosa : _ban in vitro study / _cRalph Galano, Angela Japson, Inah Krizia Lague, Jyrah Mee Makinano, Khylla Gayle Mondarte, Matt Markel Mopon, Lance Morquianos, Ma. Lucia Beatriz Mose, Timothy Bryan Ngo, Jannie Lyka Omictin, Cathelaine Diane Ong, Isabela Louise Perilla, Miguel Luis Quimbo.
260			_aSubangdaku, Mandaue City : _bUniversity of Cebu - College of Medicine Foundation, Inc., _c2026.
300			_aiv, 106 pages : _bcolor illustrations ;
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
504			_aIncludes bibliographical references.
520			_a"The emergence of multidrug-resistant Pseudomonas aeruginosa necessitates alternative antimicrobial strategies, such as quorum sensing (QS) inhibition, which targets virulence without exerting selective survival pressure. This study evaluated the anti-quorum sensing and antibiofilm activity of Psidium quajava L. methanolic leaf extracts against P. aeruginosa in vitro. Leaves collected from Medellin, Cebu, were extracted via methanolic maceration, yielding a 5.493% crude extract. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using a resazurin-based microplate broth dilution assay. Anti-QS activity was quantitatively assessed by measuring biofilm inhibition at sub-inhibitory concentrations using a crystal violet assay, with cinnamaldehyde as the positive control. The extract exhibited poor direct antimicrobial activity, recording an MIC of 12.5 mg/mL and an MBC of >12.5 mg/mL. However, it demonstrated potent, dose-dependent antibiofilm efficacy. At the baseline MIC (12.5 mg/mL), the extract achieved a 96.93% biofilm inhibition rate, remaining significantly effective at 87.68% (6.25 mg/mL) and 73.20% (3.125 mg/mL). Welch's t-test revealed the extract performs comparably to the commercial standard, cinnamaldehyde ($p = 0.408$). Furthermore, Welch's ANOVA indicated maximum statistical efficacy was achieved at 6.25 mg/mL, with no significant difference in inhibition compared to the maximum dose. These findings establish that while P. guajava methanolic leaf extract possesses weak bactericidal effects, it is a highly capable anti-quorum sensing agent that effectively disrupts biofilm formation, offering a promising alternative therapeutic strategy against antibiotic-resistant infections." --Abstact
521			_aAdult
541			_xMontoya, Ma. Melfer _ySchool of Medicine _zMicrobiology
541			_xMontoya, Ma. Melfer _ySchool of Medicine _zMicrobiology
546			_aText in English
650			_aBacterial infection.
650			_aAntimicrobial resistance.
650			_aPseudomonas aeruginosa.
650			_aMultidrug-resistant (MDR).
650			_aHospital acquired infection.
650			_aAnti-quorum sensing.
650			_aPsidium guajava L.
650			_aDrug resistant bacteria.
700			_aJapson, Angela B., _eauthor.
700			_aLague, Inah Krizia O., _eauthor.
700			_aMakinano, Jyrah Mee T., _eauthor.
700			_aMondarte, Khylla Gayle, _eauthor.
700			_aMopon, Matt Markel, _eauthor.
700			_aMarquianos, Lance, _eauthor.
700			_aMose, Ma. Lucia Beatriz, _eauthor.
700			_aNgo, Timothy Bryan, _eauthor.
700			_aOmictin, Jannie Lyka, _eauthor.
700			_aOng, Cathelaine Diane, _eauthor.
700			_aPerilla, Isabela Louise, _eauthor.
700			_aQuimbo, Miguel Luis, _eauthor.
942			_2ddc _cBK
998			_cVanessa [new] _d09/18/2026
998			_cVanessa [added] _d09/21/2026
999			_c15680 _d15680